RNA-Seq of Lolium perenne: Inflorescence primordium, Plant 11, High t... - SRA

SRX10993593: RNA-Seq of Lolium perenne: Inflorescence primordium, Plant 11, High transmission
1 ILLUMINA (Illumina HiSeq 2500) run: 88.9M spots, 22.2G bases, 7.2Gb downloads

Design: Samples (~50 mg) were powdered in liquid nitrogen using a micropestle in a 1.5 mL tube. The RNA extraction method used was a combination of an organic extraction from each sample using Trizol (Thermo Fisher Scientific, Waltham, USA) followed by further purification of 200 L of the Trizol/chloroform aqueous phase with an RNeasy MinElute Cleanup Kit (Qiagen, Valencia, USA). A TruSeq stranded mRNA sample preparation kit (Illumina, San Diego, CA, USA) was used to generate sequencing libraries from the total RNA extractions, and the indexed libraries were pooled and sequenced on two lanes of an Illumina HiSeq 2500 instrument at New Zealand Genomics Limited, Otago, New Zealand.

Submitted by: AgResearch NZ Limited

Study: Plant disease resistance mechanisms are associated with the efficiency of Epichloe endophyte seed transmission in Lolium perenne

PRJNA732816 • SRP321377 • All experiments • All runs

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Endophyte transmission in perennial ryegrass is important for plant survival and commercial reasons but the underlying biological processes are poorly understood. We aim to understand the underlying biological processes of endophyte transmission in high- and low-transmitting perennial ryegrass by using transcriptomics and other methods.

Sample: Plant 11 inflorescence primordium

SAMN19341594 • SRS9067979 • All experiments • All runs

Organism: Lolium perenne

Library:

Name: IP_11H

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 88.9M spots, 22.2G bases, 7.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR14655087	88,853,890	22.2G	7.2Gb	2022-03-03

ID:: 14621730

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